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RESEARCH PRODUCT
Morphogenesis and Cardenolide Formation in Digitalis obscura Cultures
M. C. BrisaPedro Pérez-bermúdezJ. M. FalcóJuan Segurasubject
PhysiologyCallus formationfungiDigitalis obscurafood and beveragesPlant ScienceBiologybiology.organism_classificationHypocotylTissue culturechemistry.chemical_compoundMurashige and Skoog mediumchemistryBiochemistryShootBotanyCardenolideDigoxigeninAgronomy and Crop Sciencedescription
Summary Cardenolide formation was investigated in hypocotyl cultures of Digitalis obscura L. established on MS medium with growth regulators selected to promote either callus formation, caulogenesis, embryogenesis or rhizogenesis. Examination of the cardenolide composition of leaves from mature plants was also performed. Several compounds of series A and C were identified by HPLC in in vitro and in vivo grown tissues, no marked differences being found between their cardenolide patterns. RIA determinations showed that shoot- or embryo-forming calli accumulated significantly higher amounts of digoxigenin derivatives than undifferentiated or rhizogenic cultures. In any case, field plants presented the highest contents of these metabolites.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1991-03-01 | Journal of Plant Physiology |