6533b856fe1ef96bd12b2725
RESEARCH PRODUCT
Cloning and Sequencing of a cDNA Encoding a Larval-Pupal-Specific Cuticular Protein in Tenebrio Molitor (Insecta, Coleoptera). Developmental Expression and Effect of a Juvenile Hormone Analogue
Aleth LemoineAnnie CourrentBrigitte QuennedeyIsabelle RondotJean Delachambresubject
DNA Complementaryanimal structuresmedia_common.quotation_subjectCuticleMolecular Sequence DataGenes InsectIn situ hybridizationBiologyBiochemistryComplementary DNAGene expressionAnimalsAmino Acid SequenceRNA MessengerCloning MolecularMetamorphosisTenebrioIn Situ HybridizationDNA Primersmedia_commonCloningExpression vectorBase SequenceSequence Homology Amino AcidfungiMetamorphosis BiologicalPupaGene Expression Regulation DevelopmentalProteinsMolecular biologyJuvenile HormonesLarvaJuvenile hormonedescription
A cDNA clone encoding a larval-pupal cuticular protein, named TMLPCP-22, has been isolated by screening a library in expression vector with a monoclonal antibody made against pupal cuticular proteins of Tenebrio molitor. Northern-blot and in situ hybridization analyses showed that the expression of TMLPCP-22 is regulated in a stage-specific and tissue-specific manner; the transcript was present during the secretion of preecdysial larval and pupal cuticles and was restricted to epidermal cells. No expression was observed during adult cuticle deposition. In supernumerary pupae obtained after application of a juvenile hormone analogue, which is known to inhibit the adult programme, TMLPCP-22 mRNA was expressed again, confirming its larval-pupal specificity.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1996-01-15 | European Journal of Biochemistry |