6533b856fe1ef96bd12b3041

RESEARCH PRODUCT

Biocompatibility and internalization assessment of bare and functionalised mesoporous silica nanoparticles

Pilar ErolesM. Dolores MarcosGuadalupe HerreraIris Garrido-canoRamón Martínez-máñezJuan Miguel CejalvoAna LluchFélix SancenónVicente Candela-noguera

subject

Membrane potentialBiocompatibilityToxicitymedia_common.quotation_subjectMesoporous silica nanoparticlesQUIMICA INORGANICANanoparticleGeneral ChemistryPolyethylene glycolMesoporous silicaCondensed Matter PhysicsEndocytosischemistry.chemical_compoundQUIMICA ORGANICAchemistryMechanics of MaterialsHyaluronic acidBIOQUIMICA Y BIOLOGIA MOLECULARBiophysicsGeneral Materials ScienceBiocompatibilityInternalizationmedia_commonInternalization

description

[EN] We report herein an evaluation of the effect of several mesoporous silica nanoparticles (MSNs) on the cellular uptake and in vitro cytotoxicity in human cells. Bare MSNs and MSNs functionalized with polyethylene glycol or hyaluronic acid are employed to evaluate uptake efficiency and mechanisms of endocytosis in cancer (MDA-MB-231) and non-cancer (MCF10A) cells. Moreover, changes in viability, cell cycle, oxidative stress, and mitochondrial membrane potential are evaluated. Our results confirm that MSNs are internalized efficiently by human cells and that uptake mechanisms differ for cell types and particles. We also confirm that MSNs are biocompatible materials that do not induce ROS/RNS production, nor changes on mitochondrial membrane potential or cell cycle.

yearjournalcountryeditionlanguage
2021-01-01
https://fundanet.cipf.es/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=3631