6533b856fe1ef96bd12b3092
RESEARCH PRODUCT
Cloning and characterization of the phenylalanyl-tRNA synthetase β subunit gene fromCandida albicans
Maria Gomez-loboEulogio ValentínRafael SentandreuPedro CaballeroAntonio MarcillaClaudia Pallottisubject
Base SequencebiologyGenes FungalMolecular Sequence DataSaccharomyces cerevisiaeNucleic acid sequenceRNAMolecular cloningbiology.organism_classificationMicrobiologyMolecular biologyCorpus albicansBlotting SouthernOpen reading frameBiochemistryCell WallCandida albicansGeneticsPhenylalanine-tRNA LigaseAmino Acid SequenceCloning MolecularCandida albicansMolecular BiologyGenedescription
A Candida albicans expression library was constructed from RNA isolated from regenerating protoplasts. A 1.4-kb cDNA clone was used to isolate a genomic fragment. Sequence analysis revealed an open reading frame of 593 amino acids with an overall identity of 63.6% with the phenylalanyl-tRNA synthetase beta subunit (FRS1) of Saccharomyces cerevisiae. We named it CaFRS1. It is located in a single copy in chromosome R, SfiI fragment M. Its expression showed a decrease during the cell wall regeneration process in protoplasts of both yeast and mycelial cells of C. albicans, suggesting its requirement thereof in initial steps of the cell wall synthesis.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1998-04-30 | FEMS Microbiology Letters |