6533b856fe1ef96bd12b3096
RESEARCH PRODUCT
Against Expectations: Unassisted RNA Adsorption onto Negatively Charged Lipid Bilayers
Frank DepoixMark HelmDirk SchneiderStefanie PannwittKaouthar Slamasubject
Surface PropertiesLipid BilayersFluorescence Polarization02 engineering and technology010402 general chemistry01 natural sciencesAdsorptionElectrochemistryGeneral Materials ScienceParticle SizeLipid bilayerSpectroscopyChemistryfungiPeripheral membrane proteinfood and beveragesRNABiological membraneSurfaces and Interfaces021001 nanoscience & nanotechnologyCondensed Matter PhysicsSmall molecule0104 chemical sciencesMembraneBiophysicsRNAAdsorption0210 nano-technologyhuman activitiesdescription
The composition and physicochemical properties of biological membranes can be altered by diverse membrane integral and peripheral proteins as well as by small molecules, natural and synthetic. Diverse oligonucleotides have been shown to electrostatically interact with cationic and bivalent ion loaded zwitterionic liposomes, leading to the formation of oligonucleotide-liposome aggregates. However, interaction of RNAs with other membrane surfaces remains ill understood. We used the nonnatural RNA10 to investigate RNA binding to anionic and net-uncharged membrane surfaces. RNA10 had initially been selected in a screen for nonnatural RNA motives that bind to phosphatidylcholine liposomes in the presence of Mg
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2019-10-18 | Langmuir |