6533b856fe1ef96bd12b318e
RESEARCH PRODUCT
Metabolism of Linoleic Acid or Mevalonate and 6-Pentyl-α-Pyrone Biosynthesis by Trichoderma Species
Maurice BensoussanY. HathoutLeobardo Serrano-carreónJean-marc Belinsubject
chemistry.chemical_classificationEcologybiologyStereochemistryLinoleic acidTrichoderma virideTrichoderma harzianumMycologyMetabolismbiology.organism_classificationApplied Microbiology and BiotechnologyPyronechemistry.chemical_compoundBiosynthesischemistryBiochemistryIncubationLactoneFood ScienceBiotechnologydescription
The understanding of the biosynthetic pathway of 6-pentyl-α-pyrone in Trichoderma species was achieved by using labelled linoleic acid or mevalonate as a tracer. Incubation of growing cultures of Trichoderma harzianum and T. viride with [U- 14 C]linoleic acid or [5- 14 C]sodium mevalonate revealed that both fungal strains were able to incorporate these labelled compounds (50 and 15%, respectively). Most intracellular radioactivity was found in the neutral lipid fraction. At the initial time of incubation, the radioactivity from [ 14 C]linoleic acid was incorporated into 6-pentyl-α-pyrone more rapidly than that from [ 14 C]mevalonate. No radioactivity incorporation was detected in 6-pentyl-α-pyrone when fungal cultures were incubated with [1- 14 C]linoleic acid. These results suggested that β-oxidation of linoleic acid was a probable main step in the biosynthetic pathway of 6-pentyl-α-pyrone in Trichoderma species.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1993-09-01 | Applied and Environmental Microbiology |