Miežu (Hordeum vulgare L.) mutanta nec1 transkriptoma analīze

6533b856fe1ef96bd12b34ac

RESEARCH PRODUCT

Miežu (Hordeum vulgare L.) mutanta nec1 transkriptoma analīze

Dagnija Tupiņa

subject

description

nec1 ir miežu nekrotiskais mutants, kuram ir mutācija ciklisko nukleotīdu regulētā jonu kanāla 4 (CNGC4) gēnā. nec1 ir raksturīgs HR līdzīgs fenotips, palielināts auksīna un salicilskābes daudzums, pastiprināta PR-1 un β-glukanāzes gēnu ekspresija, izmainīta koleoptiļu un atvārsnīšu atbilde uz auksīnu. nec1 transkriptoma analīze izmantojot Affymetrix miežu gēnu čipu parādīja, ka nec1 ir pastiprināta triptofāna un auksīna biosintēze, par kuru visticamāk atbildīgs indol-3-acetamīda biosintēzes ceļš, kā arī pastiprināts salicilskābes metabolisms un biosintēze. nec1 transkriptoma analīze norāda arī uz citu fitohormonu – etilēna un jasmonāta sintēzes izmaiņām. Par nec1 fenotipu atbildīgā mutācija izraisījusi arī jonu homeostāzes izmaiņas un izmaiņas sekundāro metabolītu, kas saistīti ar auga aizsargreakcijām – fenilpropanoīdu, lignīnu un flavonoīdu – biosintēzē. Dažas no nec1 transkriptoma analīzē redzamajām metabolisma izmaiņām varētu būt raksturīgas arī citiem nekrotiskajiem mutantiem.

year	journal	country	edition	language
2013-01-01

https://dspace.lu.lv/dspace/handle/7/20947

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