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RESEARCH PRODUCT
The cost of replication fidelity in an RNA virus
Victoria FurióAndrés MoyaRafael Sanjuánsubject
GeneticsMutation rateMultidisciplinaryAdaptation BiologicalRNARNA virusDNA-Directed RNA PolymerasesBiological SciencesBiologyVesicular stomatitis Indiana virusVirus Replicationbiology.organism_classificationVirologyVesicular stomatitis Indiana viruschemistry.chemical_compoundAmino Acid SubstitutionchemistryViral replicationVesicular stomatitis virusRNA polymeraseMutation (genetic algorithm)Mutagenesis Site-DirectedSelection Geneticdescription
It is often argued that high mutation rates are advantageous for RNA viruses, because they confer elevated rates of adaptation. However, there is no direct evidence showing a positive correlation between mutation and adaptation rates among RNA viruses. Moreover, theoretical work does not argue in favor of this prediction. We used a series of vesicular stomatitis virus clones harboring single amino acid substitutions in the RNA polymerase to demonstrate that changes inducing enhanced fidelity paid a fitness cost, but that there was no positive correlation between mutation an adaptation rates. We demonstrate that the observed mutation rate in vesicular stomatitis virus can be explained by a trade-off between replication rate and replication fidelity.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2005-07-08 | Proceedings of the National Academy of Sciences |