Diversity and technological potential of lactic acid bacteria of wheat flours

6533b857fe1ef96bd12b3b2c

RESEARCH PRODUCT

Diversity and technological potential of lactic acid bacteria of wheat flours

Raimondo Gaglio Antonio Alfonzo Giusi Ventimiglia Nicola Francesca Luca Settanni Giancarlo Moschetti Rosalia Di Gerlando Onofrio Corona

subject

Weissella Lactococcus Flour Leuconostoc pseudomesenteroides medicine.disease_cause Microbiology Microbiology Acidification Wheat flour Industrial Microbiology Bacterial Proteins Leuconostoc citreum Lactobacillus medicine Lactic acid bacteria Leuconostoc Lactic Acid Weissella cibaria Proteolysi Acidification; Lactic acid bacteria; Proteolysis; Sourdough; Volatile organic compounds; Wheat flour Phylogeny Triticum biology food and beverages Biodiversity Settore AGR/15 - Scienze E Tecnologie Alimentari Volatile organic compound biology.organism_classification Lactobacillaceae Sourdough Pediococcus Acids Peptide Hydrolases Food Science Settore AGR/16 - Microbiologia Agraria

description

Abstract Lactic acid bacteria (LAB) were analysed from wheat flours used in traditional bread making throughout Sicily (southern Italy). Plate counts, carried out in three different media commonly used to detect food and sourdough LAB, revealed a maximal LAB concentration of approximately 4.75 Log CFU g−1. Colonies representing various morphological appearances were isolated and differentiated based on phenotypic characteristics and genetic analysis by randomly amplified polymorphic DNA (RAPD)-PCR. Fifty unique strains were identified. Analysis by 16S rRNA gene sequencing grouped the strains into 11 LAB species, which belonged to six genera: Enterococcus, Lactobacillus, Lactococcus, Leuconostoc, Pediococcus and Weissella. Weissella cibaria, Lactobacillus plantarum, Leuconostoc pseudomesenteroides and Leuconostoc citreum were the most prevalent species. The strains were not geographically related. Denaturing gradient gel electrophoresis (DGGE) analysis of total DNA of flour was used to provide a more complete understanding of the LAB population; it confirmed the presence of species identified with the culture-dependent approach, but did not reveal the presence of any additional LAB species. Finally, the technological characteristics (acidifying capacity, antimicrobial production, proteolytic activity, organic acid, and volatile organic compound generation) of the 50 LAB strains were investigated. Eleven strains were selected for future in situ applications.

year	journal	country	edition	language
2013-01-01

10.1016/j.fm.2013.07.003 http://hdl.handle.net/10447/94533