6533b857fe1ef96bd12b430e
RESEARCH PRODUCT
Cloning and characterization of the peroxisomal acyl CoA oxidaseACO3 gene from the alkane-utilizing yeastYarrowia lipolytica
Jean-marc BelinAnnick Le ClaincheClaudie GaillardinMarie-thérèse Le DallYves WachéJean-marc NicaudHuijie J. WangYves Pagotsubject
CloningOxidase testStrain (chemistry)BioengineeringYarrowiaBiologybiology.organism_classificationApplied Microbiology and BiotechnologyBiochemistryMolecular biologyYeastBiochemistryGene expressionGeneticsAcyl-CoA oxidaseGeneBiotechnologydescription
The ACO3 gene, which encodes one of the acyl-CoA oxidase isoenzymes, was isolated from the alkane-utilizing yeast Yarrowia lipolytica as a 10 kb genomic fragment. It was sequenced and found to encode a 701-amino acid protein very similar to other ACOs, 67.5% identical to Y. lipolytica Aco1p and about 40% identical to S. cerevisiae Pox1p. Haploid strains with a disrupted allele were able to grow on fatty acids. The levels of acyl-CoA oxidase activity in the ACO3 deleted strain, in an ACO1 deleted strain and in the wild-type strain, suggested that ACO3 encodes a short chain acyl-CoA oxidase isoenzyme. This narrow substrate spectrum was confirmed by expression of Aco3p in E. coli.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1998-11-01 | Yeast |