6533b857fe1ef96bd12b463a
RESEARCH PRODUCT
Imaging Noncanonical Autophagy and LC3-Associated Phagocytosis in Cultured Cells
Oliver FloreyKatherine FletcherElise JacquinElise Jacquinsubject
0303 health sciencesChemistryATG8PhagocytosisAutophagyImmunofluorescence MicroscopyATG8 Proteins3. Good healthCell biologyBlot03 medical and health sciencesDrug treatment0302 clinical medicinePhagocytosisLAPFluorescence microscopeLC3Noncanonical autophagy[SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular BiologyATG8030217 neurology & neurosurgery030304 developmental biologydescription
International audience; Monitoring of ATG8 proteins by western blotting and immunofluorescence microscopy are the most common methods to monitor the autophagy pathway. However, it has recently been shown that ATG8 proteins can be lipidated to non-autophagosome, single-membrane compartments through a noncanonical autophagy pathway. This is commonly found to occur during macro-endocytic processes such as phagocytosis, where it has been termed LC3-associated phagocytosis, and upon lysosomotropic drug treatment. Therefore, care is required when interpreting data based on ATG8 in order to conclude whether a signal relates to the canonical or noncanonical pathway. Here we provide methods to monitor noncanonical autophagy through fluorescence microscopy.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2019-01-05 |