Determination of acetazolamide in human urine samples by reversed-phase high-performance liquid chromatography in the presence of xanthines.

6533b857fe1ef96bd12b473a

RESEARCH PRODUCT

Determination of acetazolamide in human urine samples by reversed-phase high-performance liquid chromatography in the presence of xanthines.

Rosa Herráez-hernández Pilar Campí Ns-falcó Adela Sevillano-cabeza

subject

Reproducibility Chromatography Chemistry Extraction (chemistry)Ethyl acetate General Chemistry Reversed-phase chromatography Urine High-performance liquid chromatography Acetazolamide chemistry.chemical_compound Xanthines medicine Humans Acetazolamide Drug Contamination Quantitative analysis (chemistry)Chromatography High Pressure Liquid medicine.drug

description

A simple, rapid and selective high-performance liquid chromatographic assay for the determination of acetazolamide in urine samples is described. After extraction with ethyl acetate, the drug is chromatographed on an HP-Hypersil ODS-C18 column with a mobile phase of acetonitrile-phosphate buffer (pH 3) and ultraviolet detection at 275 nm. The efficiency of the extraction, the linearity and the reproducibility of the method permit the evaluation of acetazolamide urinary excretion a long time after its administration.

year	journal	country	edition	language
1992-11-01	Journal of chromatography

10.1016/0378-4347(92)80317-j https://pubmed.ncbi.nlm.nih.gov/1491038

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