6533b857fe1ef96bd12b4df9

RESEARCH PRODUCT

Model for Generation of Large Numbers of Primary, Inflammatory Skin-Derived Neutrophils, and Macrophages

Dominik JohnMichael R. FischerEsther Von Stebut

subject

Primary (chemistry)Granuloma formationInnate immune systemIn vivoImmunologyMurine skinBiologyIsolation (microbiology)In vitro

description

Tissue macrophages and inflammatory neutrophils represent important cells of the innate immune system responsible for various important tasks, i.e., elimination of pathogens and/or granuloma formation. Isolation of large numbers of primary phagocytes is vital for research with these cells. Within this protocol, we present a strategy for isolation of large numbers of inflammatory neutrophils and macrophages from murine skin that allows for follow-up in vivo or in vitro studies.

yearjournalcountryeditionlanguage
2012-10-20
https://doi.org/10.1007/978-1-62703-227-8_27