6533b857fe1ef96bd12b4f78
RESEARCH PRODUCT
Glutathione metabolism under the influence of hydroperoxides in the lactating mammary gland of the rat. Effect of glucose and extracellular ATP.
Juan R. Vin˜aJosé M. EstrelaJuan MontoroJose Viñasubject
GPX1medicine.medical_specialtyGPX3Glutathione reductaseBiophysicsBiologyIn Vitro TechniquesBiochemistryPeroxideExcretionchemistry.chemical_compoundAdenosine TriphosphateMammary Glands Animaltert-ButylhydroperoxidePregnancyInternal medicinemedicineExtracellularAnimalsLactationMolecular BiologyRats Inbred StrainsCell BiologyGlutathioneGlutathionePeroxidesRatsMetabolic pathwayEndocrinologyGlucosechemistryBiochemistryFemaledescription
Tert-butyl hydroperoxide decreases GSH and total free glutathione (GSH+2GSSG) contents of acini from lactating mammary glands. The decrease in total free glutathione can be explained by an increase in mixed disulfide formation and by excretion of GSS G to the extracellular medium, and subsequent degradation catalyzed by gamma-glutamyl transpeptidase. Low concentrations of glucose prevented the changes in glutathione levels induced by the peroxide. In the presence of extracellular ATP, glucose did not prevent these changes. However, incubations with the peroxide, did not alter the rate of other metabolic pathways by acini.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1987-01-01 | Bioscience reports |