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RESEARCH PRODUCT
The First Cell Division Cycle in Nicotiana Mesophyll Protoplasts Cultured in vitro II. Microtubules and the Plane of Division
Wolfgang WernickeG. JungM. Rössubject
Physiologyfungifood and beveragesPlant ScienceCell plateCell cycleBiologyPhragmoplastPhragmosomeCell biologyMicrotubuleCytoplasmCytoskeletonAgronomy and Crop ScienceCytokinesisdescription
Summary Reorganization of the microtubular cytoskeleton prior to and during the first cell division cycle of Nicotiana mesophyll protoplasts was investigated using immunofluorescence microscopy. Bands of microtubules reminiscent of PPBs were found. However, their structure was comparatively diffuse. In addition, frequencies were low compared to those in meristem cells of organized tissues. Abnormalities during phragmoplast and cell plate formation were observed. The question is raised whether the formation of abnormal PPBs is related to strategic difficulties in co-ordinating the formation of a coherent plate of cytoplasm (phragmosome) traversing the vacuole to permit smooth bisecting of the vacuolated, dedifferentiating protoplasts during cytokinesis.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1991-06-01 | Journal of Plant Physiology |