6533b858fe1ef96bd12b627e

RESEARCH PRODUCT

Liquid chromatographic procedure for the evaluation of β-blockers in pharmaceuticals using hybrid micellar mobile phases

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Abstract A reversed-phase chromatographic procedure with a micellar eluent is proposed for the determination of several β-blockers (acebutolol, atenolol, carteolol, celiprolol, labetalol, metoprolol, nadolol, oxprenolol, propranolol, and timolol) in pharmaceutical formulations (tablets, capsules, and ophthalamic solutions). A study is shown on the chromatographic behaviour of these drugs with mobile phases containing sodium dodecyl sulphate (0.075–0.15 M ) and propanol (0–15%, v/v), at different pH values (3–7). The excellent correlation between log of the octanol-water partition coefficient and log of capacity factor, for the ten drugs in mobile phases of SDS and propanol, suggested that the difference in retention among them was mainly governed by their hydrophobicity. A mobile phase of 0.15 M SDS-15% propanol at pH 3 permitted the elution of the β-blockers in less than 15 min. The recoveries were usually in the 96–103% range, and the variation coefficients were below 2.5%. The results were compared with those obtained with hydro-organic eluents of methanol-phosphate buffer.