Interaction of an odorant lactone with model phospholipid bilayers and its strong fluidizing action in yeast membrane

6533b858fe1ef96bd12b644c

RESEARCH PRODUCT

Interaction of an odorant lactone with model phospholipid bilayers and its strong fluidizing action in yeast membrane

Yves Waché Mario Aguedo Laurent Beney Jean-marc Belin

subject

Time Factors [SDV.BIO]Life Sciences [q-bio]/Biotechnology Lipid Bilayers Yarrowia MESH : Models Biological Lactones MESH : Spectroscopy Fourier Transform Infrared MESH: Dimyristoylphosphatidylcholine chemistry.chemical_compound MESH : Dimyristoylphosphatidylcholine Spectroscopy Fourier Transform Infrared Membrane fluidity Organic chemistry MESH : Anti-Bacterial Agents [INFO.INFO-BT]Computer Science [cs]/Biotechnology Antibacterial agent MESH : Spectrometry Fluorescence biology MESH: Lipid Bilayers MESH: Indicators and Reagents food and beverages General Medicine Anti-Bacterial Agents MESH : Lactones Membrane Benzyl alcohol Dimyristoylphosphatidylcholine [ INFO.INFO-BT ] Computer Science [cs]/Biotechnology MESH: Lactones MESH: Spectrometry Fluorescence MESH : Time Factors MESH : Yarrowia Phospholipid [SDV.BC]Life Sciences [q-bio]/Cellular Biology Models Biological Microbiology MESH: Spectroscopy Fourier Transform Infrared MESH : Indicators and Reagents MESH: Anti-Bacterial Agents [ SDV.BC ] Life Sciences [q-bio]/Cellular Biology MESH: Time Factors MESH: Models Biological [ SDV.BIO ] Life Sciences [q-bio]/Biotechnology Yarrowia Biological membrane biology.organism_classification Yeast Spectrometry Fluorescence chemistry Indicators and Reagents MESH: Yarrowia MESH : Lipid Bilayers Food Science

description

International audience; Some odorant lactones are naturally present in fruits or in fermented products; they can also be used as food additives and can be produced by microorganisms at the industrial scale by biotechnological processes. Gamma-decalactone was previously shown to have antimicrobial properties. We determined by infrared spectroscopy measurements that this compound rapidly diffused into model phospholipid bilayers (within 2 min), modifying the general physical state of a dimyristoyl-L-alpha-phosphatidylcholine (DMPC) film. In vivo, the lactone strongly increased membrane fluidity in the model yeast Yarrowia lipolytica, as evaluated by fluorescence anisotropy measurements. This effect was more important than that of benzyl alcohol, which is known as a fluidizing agent in living cells, and may explain the toxic action of gamma-decalactone in microorganisms.

year	journal	country	edition	language
2003-02-15	International Journal of Food Microbiology

https://doi.org/10.1016/s0168-1605(02)00150-2