6533b858fe1ef96bd12b6cba

RESEARCH PRODUCT

Functional variants of 5S rRNA in the ribosomes of common sea urchin Paracentrotus lividus

Fabio CaradonnaEleonora CasconeEufrosina DimarcoDaniele Bellavia

subject

Molecular Sequence DataDNA RibosomalPolymerase Chain ReactionRibosomeParacentrotus lividusSea urchin Paracentrotus lividus 5S gene 5S rRNA variants Single-strand conformation polymorphism (SSCP)5S ribosomal RNAchemistry.chemical_compoundSequence Homology Nucleic Acidbiology.animalGeneticsAnimalsCloning MolecularInternal transcribed spacerSea urchinGenePolymorphism Single-Stranded ConformationalGeneticsBase SequencebiologyRNA Ribosomal 5SComputational BiologyGeneral MedicineNon-coding RNAbiology.organism_classificationSettore BIO/18 - GeneticachemistryOocytesParacentrotusRibosomesDNA

description

Abstract We have previously reported a molecular and cytogenetic characterization of three different 5S rDNA clusters in the sea urchin Paracentrotus lividus ; this study, performed at DNA level only, lends itself as starting point to verify that these clusters could contain transcribed genes, then, to demonstrate the presence of heterogeneity at functional RNA level, also. In the present work we report in P. lividus ribosomes the existence of several transcribed variants of the 5S rRNA and we associate all transcribed variants to the cluster to which belong. Our finding is the first demonstration of the presence of high heterogeneity in functional 5S rRNA molecules in animal ribosomes, a feature that had been considered a peculiarity of some plants.

yearjournalcountryeditionlanguage
2012-10-01Gene
https://doi.org/10.1016/j.gene.2012.07.067