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RESEARCH PRODUCT
Surface plasmon resonance signal enhancement based on erlotinib loaded magnetic nanoparticles for evaluation of its interaction with human lung cancer cells
Ayuob AghanejadSaeideh Mohammadzadeh-aslJafar Ezzati Nazhad DolatabadiMiguel De La GuardiaAhmad Keshtkarsubject
A549 cellbiologyChemistry010401 analytical chemistry02 engineering and technologyConjugated system021001 nanoscience & nanotechnology01 natural sciencesSmall moleculeAtomic and Molecular Physics and Opticsrespiratory tract diseases0104 chemical sciencesElectronic Optical and Magnetic MaterialsCancer cellmedicinebiology.proteinBiophysicsMagnetic nanoparticlesErlotinibEpidermal growth factor receptorElectrical and Electronic EngineeringSurface plasmon resonance0210 nano-technologymedicine.drugdescription
Abstract Surface plasmon resonance (SPR) sensor provides a very useful tool based on its label-free, real-time monitoring and low price properties. However, measurement of small molecules and extremely diluted analytes is difficult and therefore, signal enhancement is required. In the present study, signal enhancement of erlotinib conjugated magnetic nanoparticles (erlotinib-MNPs) compared to erlotinib was evaluated via their interaction with overexpressed epidermal growth factor receptor on human lung cancer cells (A549 cell line) surface using SPR sensor at three temperature levels. The attained results showed an average signal amplification of about 2.5-fold for MNP-erlotinib interaction with A549 cells compared to erlotinib interaction with those cells. Overall, this signal enhancement method could be used for measurement of small molecules and cancer cell detection via interaction of their overexpressed receptor and various ligands.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2021-01-01 | Optics & Laser Technology |