6533b859fe1ef96bd12b7657
RESEARCH PRODUCT
Fabrication of a Silica Coating on Magnetic γ-Fe2O3 Nanoparticles by an Immobilized Enzyme
Patrick TheatoVadim KsenofontovWolfgang TremelHelen Annal ThereseHeinz C. SchröderMartin PanthöferMuhammed Nawaz TahirWerner E.g. MüllerFilipe NatalioMohammed Ibrahim ShukoorMarc Eberhardtsubject
chemistry.chemical_classificationMaterials scienceImmobilized enzymeGeneral Chemical EngineeringBiomoleculeInorganic chemistryNitrilotriacetic acidMaghemiteNanoparticleGeneral Chemistryengineering.materialchemistry.chemical_compoundchemistryChemical engineeringMaterials ChemistryengineeringCopolymerMagnetic nanoparticlesSurface modificationdescription
Silicatein, a hydrolytic protein encountered in marine sponges, was immobilized on maghemite (γ-Fe2O3) nanoparticles that were surface functionalized with a reactive mulfunctional polymer. This polymer carries an anchor group based on dopamine which is capable of binding to the γ-Fe2O3 surface and a reactive functional group which allows binding of various biomolecules onto inorganic nanoparticles. This functional nitrilotriacetic acid (NTA) group allows immobilization of His-tagged silicatein on the surface of the γ-Fe2O3 nanoparticles. The surface-bound protein retains its native hydrolytic activity to catalyze formation of silica through copolymerization of alkoxysilanes Si(OR)4. Functionalization of the magnetic nanoparticles and the architecture of the SiO2-coated γ-Fe2O3 nanoparticles was confirmed by TEM studies as well as by FT-IR and optical microscopy.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2008-05-08 | Chemistry of Materials |