Evaluation of the DNA microarray “AMR Direct Flow Chip Kit” for detection of antimicrobial resistance genes from Gram-positive and Gram-negative bacterial isolated colonies

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RESEARCH PRODUCT

Evaluation of the DNA microarray “AMR Direct Flow Chip Kit” for detection of antimicrobial resistance genes from Gram-positive and Gram-negative bacterial isolated colonies

David Navarro Ortega Concepción Gimeno Cardona Javier Buesa Gómez Rafael Borrás Salvador Ignacio Torres Fink Nuria Tormo Palop

subject

DNA Bacterial 0301 basic medicine Microbiology (medical)030106 microbiology Gram-Positive Bacteria Sensitivity and Specificity beta-Lactam Resistance beta-Lactamases Microbiology 03 medical and health sciences 0302 clinical medicine Antibiotic resistance Bacterial Proteins Vancomycin Drug Resistance Multiple Bacterial Gram-Negative Bacteria polycyclic compounds medicine Humans 030212 general & internal medicine Gene Gram-Positive Bacterial Infections Oligonucleotide Array Sequence Analysis Gram biology Drug Resistance Microbial biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification In vitro Genes Bacterial Genetic marker Vancomycin Reagent Kits Diagnostic DNA microarray Gram-Negative Bacterial Infections Bacteria medicine.drug

description

Abstract Introduction The AMR Direct Flow Chip assay allows the simultaneous detection of a large variety of antibiotic resistance genetic markers. To assess this kit's performance, we use isolated colonies as starting material. The assay has been approved by the European Economic Area as a suitable device for in vitro diagnosis (CE IVD) using clinical specimens. Methods A total of 210 bacterial isolates harbouring either one or more antimicrobial resistance genes including plasmid-encoded extended-spectrum β-lactamases (SHV, CTX-M) and carbapenemases (GES, SME, KPC, NMC/IMI, SIM, GIM, SPM, NDM, VIM, IMP, and OXA), mecA, vanA and vanB, and 30 controls were included. Results The assay displayed a sensitivity and specificity of 100% for all target genes included in the array. Conclusion The AMR Direct Flow Chip Kit is an accurate assay for detecting genes which commonly confer resistance to β-lactams and vancomycin from isolated colonies in culture of Gram-positive and Gram-negative bacteria.

year	journal	country	edition	language
2019-08-01	Enfermedades infecciosas y microbiologia clinica (English ed.)

https://doi.org/10.1016/j.eimce.2018.12.009