Nephila clavipes flageliformā spidroīna strukturālie un biokonjugācijas pētījumi

6533b859fe1ef96bd12b7b99

RESEARCH PRODUCT

Nephila clavipes flageliformā spidroīna strukturālie un biokonjugācijas pētījumi

Rimants Metlāns

subject

ZIRNEKĻU ZĪDS KRISTALIZĒŠANA E. coli SPIDROĪNI PEGILĒŠANA Ķīmija

description

Nephila clavipes flageliformā spidroīna strukturālie un biokonjugācijas pētījumi Metlāns R., darba vadītājs zinātn. asist., Mg. biol. Jēkabs Fridmanis, darba vadītājs Dr. biol., prof. Kaspars Tārs, bakalaura darbs, 47 lappuses, 19 attēli, 3 tabulas, 47 literatūras avoti, 2 pielikumi. Latviešu valodā. Bakalaura darba literatūras apskatā apkopota vispārīga informācija par proteīniem, to uzbūvi un funkcijām, zirnekļu zīdu, tā uzbūvi, domēnu īpatnībām, proteīnu kristalizēšanas procesu un PEGilēšanas reakcijām. Bakalaura darba eksperimentālajā daļā FlagSpNT proteīnā tika ieviesta selenometionīna iezīme un tika optimizēti tā kristalizācijas apstākļi līdz tika iegūts monokristāls. Izmantojot to, zinātnieki no Latvijas Biomedicīnas pētījumu un studiju centra noteica FlagSpNT struktūru. Tika optimizēti Flag1rep proteīna ekspresijas un attīrīšanas apstākļi un veikta PEGilēšanas reakcijas optimizācija.

year	journal	country	edition	language
2021-01-01

https://dspace.lu.lv/dspace/handle/7/55146

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