Ādas 3D modeļsistēmas veidošana un raksturošana in vitro

6533b859fe1ef96bd12b7be7

RESEARCH PRODUCT

Ādas 3D modeļsistēmas veidošana un raksturošana in vitro

Liene Pirtniece

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Darba mērķis bija izveidot un raksturot in vitro ādas audu modeli, kam pamatā ir ādas šūnu – fibroblastu un keratinocītu kokultūru veidošana un kultivēšana trīsdimensionālā (3D) fibrīna matriksā. Lai sasniegtu mērķi, matriksā tika kultivētas divas dermas šūnu līnijas, kā arī no ādas izdalītu primāro keratinocītu līnija un standartizētu keratinocītu līnija (HaCat). Šūnu kokultūras tika kultivētas divas, trīs un četras nedēļas. Ādas modeļsistēmas raksturošanai tika izmantotas imunohistoķīmijas un ELISA metodes, pēc kurām tika noteikta iegūto ādas audu paraugu morfoloģija un funkcionalitāte. Iegūtie dati norāda uz to, ka izveidotās ādas šūnas audu modeļu testsistēmās ir funkcionālas. Tās producē gan fibroblastu un keratinocītu augšanas faktorus, gan metalloproteināzes. Pēc histoloģiskajām analīzēm tika parādīta šūnu spēja dalīties, augt un pielāgoties 3D fibrīna matriksā.

year	journal	country	edition	language
2013-01-01

https://dspace.lu.lv/dspace/handle/7/26888

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