Borrelia burgdorferi celma B31 virsmas proteīna BBA68 struktūras noteikšana un analīze

6533b859fe1ef96bd12b85c2

RESEARCH PRODUCT

Borrelia burgdorferi celma B31 virsmas proteīna BBA68 struktūras noteikšana un analīze

Elviss Bērtulis

subject

ārējās virsmas proteīni Laima slimība Borrelia burgdorferi Bioloģija proteīnu kristalizācija proteīnu attīrīšana

description

Borrelia burgdorferi ir spirohetu dzimtas baktērija, kura izraisa Laima slimību. Lai izvairītos no saimniekorganisma imūnajām reakcijām, B. burgdorferi ir izveidojies komplicēts gēnu regulācijas mehānisms, kur liela nozīme ir ārējās virsmas proteīniem. Darba mērķis bija ar rentgenstruktūranalīzes palīdzību noteikt B. burgdorferi celma B31 ārējā virsmas proteīna BBA68 trīsdimensiālo struktūru, un analizēt strukturālās atšķirības no B. burgdorferi celma ZS7 ārējā virsmas proteīna BbCRASP-1. Strukturālie pētījumi ļauj prognozēt proteīnu funkcijas, tādā veidā gūstot plašāku ieskatu par B. burgdorferi baktērijas mehānismiem Laima slimības patoģenēzē. Pētījumā sekmīgi tika iegūts proteīna BBA68 rekombinants, un tika veikta tā ekspresija, attīrīšana un kristalizācija.

year	journal	country	edition	language
2015-01-01

https://dspace.lu.lv/dspace/handle/7/29469

PEPhysical Sciences and Engineering
- PE2Fundamental Constituents of Matter
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