6533b859fe1ef96bd12b867a
RESEARCH PRODUCT
NiII, and ZnII Schiff Base Complexes: Telomeric G-quadruplex Stabilizers
A MarroneRiccardo BonsignoreAlessio TerenziAngelo SpinelloCarla GentileAnnamaria MartoranaAnna Maria AlmericoAntonino LauriaGiampaolo Baronesubject
Telomeric G-quadruplex Stabilizers c-Myc c-Kit Schiff base complexes Salphen-like metal complexesSettore CHIM/03 - Chimica Generale E InorganicaSettore BIO/10 - BiochimicaSettore CHIM/08 - Chimica Farmaceuticadescription
Recently, NiII and ZnII metal complexes of the ligand Salpyrim have been synthesized and characterized. Their affinity for wild-type h-Telo G-quadruplex DNA and for calf thymus DNA was investigated by UV absorption spectroscopy, circular dichroism and viscometry. The data collectively suggest that both complexes bind effectively to G-quadruplexes by direct end-stacking, stabilizing the oligonucleotide secondary structure. The two complexes are also typical B-DNA intercalators. Remarkably, their binding constants, Kb, with the G4s structures are about 10 fold higher than those with B-DNA, highlighting the selectivity. Experiments to evaluate the biological activity of the two complexes against MCF7 and HeLa cancer cell lines are currently ongoing.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2014-01-01 |