6533b85afe1ef96bd12b9470

RESEARCH PRODUCT

Macromolecules in ordered media VIII. High-performance size-exclusion chromatography as a technique for characterizing the interaction between polyanions and cationic liposomes

Rosa Garci´aIolanda PorcarIolanda PorcarConcepcio´n AbadI´ngrid Catala´Agusti´n Campos

subject

LiposomeChromatographyOrganic ChemistrySize-exclusion chromatographyFluorescence spectrometryGeneral MedicineBiochemistryPolyelectrolyteAnalytical ChemistryGel permeation chromatographychemistry.chemical_compoundchemistryIonic strengthPhosphatidylcholineAcrylic acid

description

Abstract The association of polyanions with liposomes based on dimyristoyl phosphatidyl choline and stearylamine at a ratio of 50:50 was investigated in terms of the binding model and, to a lesser extent, of the partition model. Aqueous size-exclusion chromatography has been proposed as a major technique for monitoring the interactions between liposomes and polyelectrolytes and the results were compared with those obtained by fluorescence spectroscopy. Quantitatively, the extent of the association follows the order: Sodium poly(styrene sulfonate) ( K A = 10 000 M −1 ), poly (acrylic acid) ( K A = 2000 M −1 ) and poly ( l -glutamic acid) ( K A = 700 M −1 ). Also, the effect of the ionic strength has been studied, showing an enhancement of the association as the ionic strength increases. Furthermore, a plausible way in which the interaction occurs has been proposed, based on the calculated number of phospholipids involved in the binding and on the polymer's conformational structure.

https://doi.org/10.1016/s0021-9673(97)00385-3