6533b85bfe1ef96bd12ba2dd

RESEARCH PRODUCT

Selective culture of rat CNS neurons in a synthetic medium.

Francesco VitaleDonatella FerraroR BarbieriA. CestelliGiovanni Savettieri

subject

NeuronsNonneuronal cellNeurofilamentGeneral NeuroscienceBrainDermatologyGeneral MedicineBiologyEmbryo MammalianCell biologyCulture MediaRatsPsychiatry and Mental healthChemically defined mediumnervous systemCell cultureAnimalsNeurology (clinical)NeurogliaCells Cultured

description

A Chemically Defined Medium is described which facilitates the survival of neurons in primary cultures of rat cerebral hemispheres. More than 90% of all cells were identified as neurons using neurofilament as a marker in an immunocytochemical assay. In contrast, serum-supplemented medium, by stimulating nonneuronal cell proliferation, hinders the survival of neurons in culture.

yearjournalcountryeditionlanguage
1983-09-01Italian journal of neurological sciences
10.1007/bf02043486https://pubmed.ncbi.nlm.nih.gov/6642999