6533b85bfe1ef96bd12baa1c
RESEARCH PRODUCT
Tirant is a new member of the gypsy family of retrotransposons in Drosophila melanogaster.
Joaquín CañizaresParicio NMoltóGrau Msubject
Transposable elementRetroelementsvirusesRetrotransposonGenes envOpen Reading FramesPhylogeneticsConsensus SequenceGeneticsConsensus sequenceAnimalsORFSMolecular BiologyPhylogenySouthern blotGeneticsbiologyBase SequenceTerminal Repeat SequencesGeneral Medicinebiology.organism_classificationGenes gagGenes polOpen reading frameDrosophila melanogasterDrosophila melanogasterSequence AlignmentBiotechnologydescription
In this paper, we propose a consensus sequence for a putative complete Tirant retrotransposon. Several defective copies, as well as relevant sequences available in databases have been analyzed. The putative complete Tirant element is 8533 bp long, and presents all the structural features of a retroviruslike transposable element of the gypsy family. It contains three ORFs (open reading frames) that encode putative products resembling the retroviral Gag, Pol, and Env proteins. Southern blot analyses show that complete and defective Tirant elements are widespread in Drosophila melanogaster. The different hybridization patterns observed in several natural populations of this species suggest that Tirant is an active element. Key words: Drosophila, LTR-retrotransposon, gypsy, retrovirus, envelope protein, phylogeny.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2000-03-04 | Genome |