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RESEARCH PRODUCT

Comparison between Disk Diffusion and Microdilution Methods for Determining Susceptibility of Clinical Fungal Isolates to Caspofungin

Maida Carmelo MassimoSalvatore Massimo OliveriFrancesco BarchiesiBarbara RavazzoloMaria Eleonora MiliciGiorgio ScaliseElisabetta Spreghini

subject

Microbiology (medical)Settore MED/07 - Microbiologia E Microbiologia ClinicaSusceptibility testingAntifungal AgentsInhibition zonefood.ingredientGeotrichumMicrobial Sensitivity TestsMycologyMicrobiologyDiffusionEchinocandinsLipopeptideschemistry.chemical_compoundfoodCaspofunginCandida albicansAgarCandida albicansCaspofungin Candida Disk diffusion antimicrobial testingbiologyBroth microdilutionFungibiology.organism_classificationYeastchemistryCaspofungin

description

ABSTRACT We compared the caspofungin (CAS) susceptibility testing results generated by the disk diffusion (DD) assay with the results of the Clinical and Laboratory Standards Institute (CLSI) broth microdilution (BD) reference method for 106 yeast isolates. The isolates represented 11 different fungal species, including Candida albicans ( n = 50), C. parapsilosis ( n = 10), C. glabrata ( n = 10), C. tropicalis ( n = 10), C. guillermondii ( n = 6), C. rugosa ( n = 5), C. krusei ( n = 5), C. kefyr ( n = 2), C. pelliculosa ( n = 2), Saccharomyces cerevisiae ( n = 3), and Geotrichum candidum ( n = 3). The DD assay was performed in supplemented Mueller-Hinton agar with CAS, which was tested at concentrations of 2, 10, and 25 μg per disk. MICs and inhibition zone diameters were evaluated at 24 and 48 h. In general, the results obtained by the DD assay correlated well with those obtained by the BD method. In particular, a significant correlation between methods was observed when CAS was used at concentration of 2 μg/disk at a reading time of either 24 or 48 h.

yearjournalcountryeditionlanguage
2007-11-01Journal of Clinical Microbiology
https://doi.org/10.1128/jcm.00826-07