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RESEARCH PRODUCT
Plasmonic Nanosensors for Simultaneous Quantification of Multiple Protein–Protein Binding Affinities
Germán RivasLydia TomeDirk SchneiderAndreas HenkelCarsten SönnichsenRubén Ahijado-guzmánChristina RosmanJanak Prasadsubject
Models MolecularNanotubesBacteriaChemistryMechanical EngineeringProtein proteinBioengineeringNanotechnologyGeneral ChemistrySurface Plasmon ResonanceCondensed Matter PhysicsCytoskeletal ProteinsBacterial ProteinsNanosensorProtein Interaction MappingGeneral Materials ScienceNanorodGoldPlasmonProtein BindingBinding affinitiesMacromoleculedescription
Most of current techniques used for the quantification of protein-protein interactions require the analysis of one pair of binding partners at a time. Herein we present a label-free, simple, fast, and cost-effective route to characterize binding affinities between multiple macromolecular partners simultaneously, using optical dark-field spectroscopy and individual protein-functionalized gold nanorods as sensing elements. Our NanoSPR method could easily become a simple and standard tool in biological, biochemical, and medical laboratories.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2014-09-08 | Nano Letters |