6533b85bfe1ef96bd12bb477
RESEARCH PRODUCT
Genetic variability in environmental isolates of Legionella pneumophila from Comunidad Valenciana (Spain).
Mireia CoscollaFernando González-candelasMaría José GosalbesVicente Catalánsubject
GeneticsRecombination GeneticLinkage disequilibriumGeographyPopulation structureMolecular Sequence DataOutbreakGenetic VariationSequence Analysis DNABiologybiology.organism_classificationMicrobiologyLegionella pneumophilaLinkage DisequilibriumNucleotide diversityLegionella pneumophilaSpainGenetic variationGenetic variabilityLegionnaires' DiseaseEcology Evolution Behavior and SystematicsAllelesEnvironmental Monitoringdescription
Summary Legionella pneumophila is associated to recurrent outbreaks in several Comunidad Valenciana (Spain) localities, especially in Alcoi, where social and climatic conditions seem to provide an excellent environment for bacterial growth. We have analysed the nucleotide sequences of three loci from 25 environmental isolates from Alcoi and nearby locations sampled over 3 years. The analysis of these isolates has revealed a substan- tial level of genetic variation, with consistent patterns of variability across loci, and comparable to that found in a large, European-wide sampling of clinical isolates. Among the tree loci studied, fliC showed the highest level of nucleotide diversity. The analysis of isolates sampled in different years revealed a clear differenti- ation, with samples from 2001 being significantly distinct from those obtained in 2002 and 2003. Furthermore, although linkage disequilibrium mea- sures indicate a clonal nature for population structure in this sample, the presence of some recombination events cannot be ruled out.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2006-06-01 | Environmental microbiology |