6533b85bfe1ef96bd12bbe2d

RESEARCH PRODUCT

Two-site ELISA for quantification of the terminal C5b-9 complement complex in plasma

A. Accardo-palumboG. TrioloD. CasigliaL. SallìE. GiardinaG. Triolo

subject

Anticorps monoclonalbiologymedicine.drug_classChemistryImmunologychemical and pharmacologic phenomenaMonoclonal antibodyMolecular biologyfemale genital diseases and pregnancy complicationsPolyclonal antibodiesparasitic diseasesMonoclonalmedicinebiology.proteinImmunology and AllergyRheumatoid factorComplement membrane attack complexQuantitative analysis (chemistry)Normal range

description

Abstract A quantitative ELISA procedure using monoclonal and polyclonal antibodies against neoantigens of the terminal C5b-9 complement complex has been developed. The ELISA was demonstrated to be both sensitive and reproducible. The normal range for C5b-9 determinations, defined as 2.5–97.5% interval of the values obtained in 76 healthy blood donors, was 3.12–10.3 AU/ml. The presence of rheumatoid factor did not affect the determination of C5b-9 as demonstrated by immunoabsorption studies.

yearjournalcountryeditionlanguage
1993-08-01Journal of Immunological Methods
https://doi.org/10.1016/0022-1759(93)90119-r