6533b85cfe1ef96bd12bc060

RESEARCH PRODUCT

Fast Separation and Determination of Sterols in Vegetable Oils by Ultraperformance Liquid Chromatography with Atmospheric Pressure Chemical Ionization Mass Spectrometry Detection

José Manuel Herrero-martínezAlberto MéndezMaría Jesús Lerma-garcíaErnesto F. Simó-alfonsoJosep Lluís Lliberia

subject

Detection limitChemical ionizationChromatographyAtmospheric pressureChemistryAnalytical chemistryAtmospheric-pressure chemical ionizationGeneral ChemistryMass spectrometryHigh-performance liquid chromatographyMass SpectrometrySterolschemistry.chemical_compoundAtmospheric PressureVegetable oilPlant OilsGeneral Agricultural and Biological SciencesAcetonitrileChromatography Liquid

description

A method for the determination of sterols in vegetable oils by ultraperformance liquid chromatography (UPLC) with atmospheric pressure chemical ionization mass spectrometry detection has been developed. The separation of sterols was optimized in terms of mobile phase composition, column temperature and flow rate. The optimal conditions were achieved using an Acquity UPLC BEH C18 column (50 x 2.1 mm, 1.7 microm) with a mobile phase consistent of acetonitrile/water (0.01% acetic acid) using a linear gradient, at a flow rate of 0.8 mL min(-1) and column temperature of 10 degrees C, giving a total analysis time below 5 min. The determination was performed in selective ion recording mode. The limits of detection were in all cases below 0.07 microg mL(-1), with relative standard deviation values of retention times and peak areas below 0.4 and 5%, respectively. The content of main sterols present in several vegetable oils with different botanical origins was also established.

yearjournalcountryeditionlanguage
2010-02-12Journal of Agricultural and Food Chemistry
https://doi.org/10.1021/jf9043887