A functional analysis of ACP-20, an adult-specific cuticular protein gene from the beetle Tenebrio. Role of an intronic sequence in transcriptional activation during the late metamorphic period

6533b85cfe1ef96bd12bc14c

RESEARCH PRODUCT

A functional analysis of ACP-20, an adult-specific cuticular protein gene from the beetle Tenebrio. Role of an intronic sequence in transcriptional activation during the late metamorphic period

Jean Delachambre Jocelyne Mathelin Aleth Lemoine Claude Everaerts Christine Braquart-varnier

subject

MESH : Molecular Sequence Data [ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition MESH : Genes Reporter/physiology MESH : Transcriptional Activation/genetics MESH : Introns/genetics Promoter Regions (Genetics)/drug effects/physiology Exon 0302 clinical medicine Genes Reporter Transcriptional regulation Trans-Activation (Genetics)/genetics/*physiology MESH : Tenebrio/genetics Luciferases Promoter Regions Genetic Tenebrio Peptide sequence MESH : Metamorphosis Biological/genetics ComputingMilieux_MISCELLANEOUS 0303 health sciences MESH : Amino Acid Sequence Metamorphosis Biological MESH : Luciferases/metabolism Ecdysone/metabolism/pharmacology 3. Good health Insect Proteins MESH : Transfection Sequence Analysis Signal peptide Transcriptional Activation Ecdysone animal structures Sequence analysis Molecular Sequence Data MESH : Transcriptional Activation/physiology Reporter/physiology Biological/genetics/*physiology MESH : Insect Proteins/physiology Biology Luciferases/metabolism Transfection Tenebrio/*genetics/physiology MESH : Ecdysone/pharmacology 03 medical and health sciences Genetics Animals Amino Acid Sequence [ SDV.BDD ] Life Sciences [q-bio]/Development Biology Molecular Biology Gene MESH : Introns/physiology 030304 developmental biology Gene Library MESH : Metamorphosis Biological/physiology Reporter gene [SDV.GEN.GPO]Life Sciences [q-bio]/Genetics/Populations and Evolution [q-bio.PE]Base Sequence Metamorphosis Intron Introns/genetics/physiology MESH : Ecdysone/metabolism Sequence Analysis DNA DNA MESH : Gene Library Molecular biology Introns Genes MESH : Tenebrio/physiology Epidermis/metabolism Gene Library Insect Science MESH : Insect Proteins/genetics MESH : Epidermis/metabolism MESH : Base Sequence MESH : Animals Epidermis MESH : Promoter Regions Genetic/drug effects [SDE.BE]Environmental Sciences/Biodiversity and Ecology Insect Proteins/*genetics/*physiology 030217 neurology & neurosurgery Epidermis/metabolism MESH : Promoter Regions Genetic/physiology MESH : Sequence Analysis DNA

description

0962-1075 (Print) Comparative Study Journal Article Research Support, Non-U.S. Gov't; A gene encoding the adult cuticular protein ACP-20 was isolated in Tenebrio. It consists of three exons interspersed by two introns, intron 1 interrupting the signal peptide. To understand the regulatory mechanisms of ACP-20 expression, ACP-20 promoter-luciferase reporter gene constructs were transfected into cultured pharate adult wing epidermis. Transfection assays needed the presence of 20-hydroxyecdysone, confirming that ACP-20 is up-regulated by ecdysteroids. Analysis of 5' deletion constructs revealed that three regions are necessary for high levels of transcription. Interaction experiments between intronic fragments and epidermal nuclear proteins confirmed the importance of intron 1 in ACP-20 transcriptional control, which results from the combined activity of regulatory cis-acting elements of the promoter and those of intron 1.

year	journal	country	edition	language
2004-10-01

10.1111/j.0962-1075.2004.00508.x https://hal.archives-ouvertes.fr/hal-00121278