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RESEARCH PRODUCT

Quantitative Prediction of Concentration Effects in Steric Exclusion Chromatography

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Abstract A semiempirical model, based on a previous one quantitatively describing the dependence of the elution volume, V(cA), on the concentration of injected polymer, cA, in exclusion chromatography (SEC) at dilute solutions, has been developed. In the derived equation, concentration effects are mainly governed by the Huggins' coefficient, kA, and by the quadratic coefficient in the polynomial expansion of the reduced specific viscosity, kA. Because of the incertitudes on reliable kA and kA' values, these are respectively removed from the model through she Imai's equation and the empirical correlation kA' + 0.122=kA, here obtained. Thus, A predicted elution volumes besides polymer concentration only depend on the polymer intrinsic viscosity and on its unperturbed dimensions constant,Kθ. The polymer concentration range of model applicability is up to Jerately diluted polymer solutions, as a comparison between predicted and experimental elution volumes for diverse literature systems shows.