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RESEARCH PRODUCT
Soluble E-Selectin Enhances Intercellular Adhesion Molecule-1 (ICAM-1) Expression in Human Tumor Cell Lines
Werner-johannes MayetAndreas SchwartingB M WittigT SchreiterRoman A. BlahetaUlrich TreichelK H Meyer Zum Büschenfeldesubject
ICAM3Time FactorsICAM2Cell adhesion moleculeT-LymphocytesIntercellular Adhesion Molecule-1Soluble cell adhesion moleculesGene ExpressionCell BiologyBiologyIntercellular Adhesion Molecule-1Intercellular adhesion moleculeMolecular biologyUp-RegulationCell biologySolubilityCell AdhesionTumor Cells CulturedHumansNeoplasm InvasivenessNeural cell adhesion moleculeRNA MessengerE-SelectinCell adhesiondescription
E-selectin mediates neovascularization via its soluble form, while its membrane-bound form initiates binding of tumor cells to vascular endothelium. Therefore, it was studied whether soluble E-selectin regulates further adhesion molecules on tumor cells. In tumor cells but not in related nonmalignant cells, intercellular adhesion molecule (ICAM)-1 expression was strikingly increased from 5 to 68% positive cells by in vitro inoculation of a recombinant E-selectin-IgG1 within 24 h, as analyzed by flow cytometry. The absence of changes in the expression of vascular cell adhesion molecule, integrin ligands (CD11a, CD18, integrin alpha 4), and sialyl-Lewis X indicates a specific effect of soluble E-selectin on ICAM-1. A cell adhesion assay revealed that the enhanced adhesion on T-cells to tumor cells mediated by soluble E-selectin-induced ICAM-1 expression was at a maximum after a 12-h incubation period. Therefore, ICAM-1 regulation on tumor cells might be a mechanism of immune escape.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1998-01-22 | Experimental Cell Research |