IS10H loma hloramfenikola acetiltransferāzes gēna aktivēšana Escherichia coli plazmīdu genomā kultūrstacionārajā augšanas fāzē

6533b85cfe1ef96bd12bcdb5

RESEARCH PRODUCT

IS10H loma hloramfenikola acetiltransferāzes gēna aktivēšana Escherichia coli plazmīdu genomā kultūrstacionārajā augšanas fāzē

Dmitrijs Perminovs

subject

description

IS10 ir viens no aktīvākajiem transpozoniem baktērijās. Elementam ir liela nozīme Escherichia coli genomā pārkārtošanās, kas notiek šūnu kultūrā iestājoties stacionārai augšanas fāzei. Ģenētisko izmaiņu rezultātā veidojas adaptīvās mutācijas, kas pārsvarā ir neekspresējamo gēnu aktivācijas rezultāts. Darba mērķis bija noskaidrot IS10 izraisīto nefunkcionējoša hloramfenikola acetiltransferāzes gēna aktivācijas iespējamos mehānismus. Izpētīts, ka plazmīda pAdCat ir efektīvs vektors IS10 izoformas – IS10H secības izolēšanai. Modelēts, ka bezpromotera cat varētu būt aktivizēts ar iekšējām IS10H sekvencēm, kas veido baktēriju -10 un -35 promoteriem attāli homologas secības. Noteikts, kā IS10H integrācijas sekvences atšķiras no aprakstītajām IS10R elementam. Ar DNS blotu noteiktas būtiskās atšķirības IS10 izplatībā genomā diviem tuvu radniecīgiem XL1-blue celma atvasinājumiem. Noteikta pozitīvā korelācija starp starpreplikonu transpozīcijas biežumu un elementu skaitu genomā. Intragenomiskā elementu migrēšana netika konstatēta, arī pēc ģenētiskā un metaboliskā stresa izraisīšanas šūnām.

year	journal	country	edition	language
2011-01-01

https://dspace.lu.lv/dspace/handle/7/15870

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