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RESEARCH PRODUCT
Three cell subsets are required for the transfer of delayed-type hypersensitivity reaction by antigen-specific T cell lines.
Guido SireciWlodzmierz PtakFrancesco DieliGeoffrey L. AshersonAlfredo SalernoVittorio ColizziA. Bellaviasubject
MaleCD3T cellReceptors Antigen T-Cell alpha-betaImmunologychemical and pharmacologic phenomenaPicryl ChlorideBiologyDermatitis ContactCell LineImmunophenotypingMiceImmune systemAntigenT-Lymphocyte SubsetsmedicineCytotoxic T cellAnimalsHypersensitivity DelayedAntigen-presenting cellInterleukin 4Receptors Antigen T-Cell gamma-deltaMolecular biologymedicine.anatomical_structureImmunologybiology.proteinMice Inbred CBAInterleukin-4Lymph NodesCD8Spleendescription
Antigen (trinitrochlorobenzene)-specific T cell lines were obtained by repeated stimulation of lymph node cells from immune mice with antigen in vitro. These T cell lines, consisting of more than 90% CD4+ Vbeta8.2+ and 6 to 9% gammadelta+ T lymphocytes, transfer contact sensitivity (CS) locally when injected at the same site as the challenge antigen, but fail to mediate a systemic passive transfer when injected i.v. Injection of T cell lines together with spleen cells from mice immunized 1 day beforehand (1-day cells) allowed a successful, specific systemic transfer of CS. Phenotypic analysis showed that the 1-day immune cell was alphabeta+, gammadelta-, sIg-, CD3+, CD4-, CD8-, CD5+, B220 (CD45R)+, Thy 1.2+. The effect of 1-day immune cells occurred through a mechanism involving IL-4, as 1-day immune cells failed to allow systemic transfer of CS by T cell lines in recipient mice treated with mAb to IL-4. These observations strongly indicate that three cell subsets are required for the systemic passive transfer of CS by T cell lines: alphabeta+ CD4+, gammadelta+, and a third cell subset, which is CD45R+, alphabeta+, CD3+, but double (CD4, CD8) negative.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1997-02-01 | Cellular immunology |