6533b85dfe1ef96bd12bddc9

RESEARCH PRODUCT

Genetic relationships between Sicilian wild populations of Brassica analysed with RAPD markers

Anna GeraciI. DivaretFrancesco Maria RaimondoAnne-marie Chèvre

subject

0106 biological sciencesBrassicaPopulation geneticsPlant Science01 natural sciences03 medical and health sciencesRAPDBotanyGenetics[SDV.BV]Life Sciences [q-bio]/Vegetal Biology[SDV.BV] Life Sciences [q-bio]/Vegetal BiologyGenetic variabilityComputingMilieux_MISCELLANEOUS030304 developmental biology0303 health sciencesbiologyDendrogramUPGMAAMELIORATION DES PLANTESGENETIQUE15. Life on landbiology.organism_classificationRAPDGenetic distanceGenetic markerTAXINOMIEAgronomy and Crop Science010606 plant biology & botany

description

Brassica comprises very variable species, both morphologically and genetically. Among these species, the Sicilian populations of Brassica sect. Brassica, species related to kale crops form a complex group. The genetic relationships among 15 populations occurring in Sicily and one from Calabria, representing the existing diversity, have been investigated using random amplified polymorphic DNA (RAPD) markers. This assay, carried out with 22 arbitrary primers, generated 236 polymorphic fragments, 21 of which were specific for single populations (mainly Brassica insularis, Brassica incana and Brassica macro-carpa). Jaccard's genetic distances were computed and the phylogenic tree was established using the UPGMA algorithm. The dendrogram obtained showed four branches grouping: (1) B. incana populations; (2) B. insularis and B. macrocarpa, occurring in small islands around Sicily; (3) B. rupestris populations; and (4) B. villosa populations. Within B. rupestris, only B. rupestris subsp. brevisiliqua was clustered in the B. villosa group. The classification obtained is discussed with regard to the morphological, ecological and geographical data.

yearjournalcountryeditionlanguage
2001-06-26Plant Breeding
https://doi.org/10.1046/j.1439-0523.2001.00589.x