6533b85dfe1ef96bd12bde0a

RESEARCH PRODUCT

Comparative study on biological effects of the guinea pig complement-peptide C3a and C3a-related synthetic oligopeptides

Ulrich HaddingS. BeckerStefan MeuerDieter Bitter-suermannR. AndreattaH. U. Schorlemmer

subject

Blood PlateletsCytotoxicity ImmunologicAnaphylatoxinsSerotoninContraction (grammar)ImmunologyDose-Response Relationship Immunologicchemical and pharmacologic phenomenaPeptideBiologyGuinea pigMiceAnimalsBioassayPlateletCytotoxicityMolecular Biologychemistry.chemical_classificationOligopeptideL-Lactate DehydrogenaseComplement C3Peptide Chain Termination TranslationalCytolysisBiochemistrychemistryBiological AssayOligopeptides

description

Dose-response experiments with guinea pig C3a and a synthetic hexapeptide (amino acid residues 72–77), representing the COOH-terminal sequence of human C3a, were performed in two recently described bioassay systems for C3a, i.e. cytotoxicity against tumor cells measured as LDH and 51Cr-release and non cytolytic serotonin release from guinea pig platelets. Compared to the classical anaphylatoxic assay (guinea pig ileum contraction), nearly identical reactivities were observed in all three test systems with C3a and, although quantitatively different, with hexapeptide.

yearjournalcountryeditionlanguage
1980-10-01Molecular Immunology
https://doi.org/10.1016/0161-5890(80)90022-x