6533b85dfe1ef96bd12be810
RESEARCH PRODUCT
Separation of aminoalkanephosphonic acid enantiomers by indirect UV detection capillary electrophoresis with application of cyclodextrins.
Piotr WieczorekPaweł KafarskiPaweł KafarskiEwa Rudzińskasubject
aminophosphonic acidsClinical Biochemistrycapillary electrophoresisOrganophosphonatesElectrolyteBiochemistryindirect UV detectionAnalytical ChemistryCapillary electrophoresisMolar ratiopolycyclic compoundsheterocyclic compoundsAmineschemistry.chemical_classificationCyclodextrinsChromatographyCyclodextrinorganic chemicalsElectrophoresis CapillaryStereoisomerismHydrogen-Ion ConcentrationProcess conditionschemistrycyclodextrinUv detectionEnantiomerenantiomer separationdescription
Indirect UV detection capillary electrophoresis (CE) was used for the separation of aminoalkanephosphonic acid (AP) enantiomers by applying commercially available cyclodextrins as chiral discriminators. The results show that the separation of the enantiomers depends on pH of the background electrolyte, the molar ratio of cyclodextrin to aminophosphonic acid, and on the type of the applied chiral selector. Optimization of process conditions allowed enantiomeric baseline separation or partial separation of 12 out of 14 alpha-aminophosphonic acids studied. This type of CE might therefore be successfully used for routine determination of enantiomeric purity of aminophosphonic acids.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2003-08-01 | Electrophoresis |