6533b85dfe1ef96bd12be902

RESEARCH PRODUCT

Determination of tyrosine through a FIA-direct chemiluminescence procedure.

J. Martínez CalatayudM.c. Sanfeliu AlonsoL. Lahuerta Zamora

subject

chemistry.chemical_classificationReproducibilityPotassium hydroxideChromatographyChemistryFormic acidRepeatabilityAnalytical Chemistrylaw.inventionAmino acidchemistry.chemical_compoundlawTyrosineQuantitative analysis (chemistry)Chemiluminescence

description

A new FI-direct chemiluminescence method is proposed for the determination of tyrosine, based on the oxidation of the amino acid by K(3)Fe(CN)(6) in potassium hydroxide medium, at room temperature and enhanced by the presence of beta-cyclodextrin and formic acid. The dynamic range was linear over the range 1.0-10.0 mgl(-1). A large study of the influence of foreign compounds was performed, including amino acids; and, the method showed high selectivity. The reproducibility between days resulted in a rsd (in slope%) of 4.8 and the repeatability with a rsd (n=50, 10.0 mgl(-1)) of 3.1%, the LOD (s/n=3) was 50 mugl(-1) and sample throughput 98 h(-1).

yearjournalcountryeditionlanguage
2003-06-13Talanta
10.1016/s0039-9140(03)00099-7https://pubmed.ncbi.nlm.nih.gov/18969059