6533b85efe1ef96bd12bf2ee

RESEARCH PRODUCT

Adrenergic Stimulation of Cyclic GMP Formation Requires NO-Dependent Activation of Cytosolic Guanylate Cyclase in Rat Pinealocytes

Lutz VollrathElisabeth LayesRainer Spessert

subject

medicine.medical_specialtyGUCY1B3AdrenergicStimulationArginineNitric OxidePineal GlandBiochemistryPinealocyteNitric oxideCellular and Molecular Neurosciencechemistry.chemical_compoundCytosolInternal medicinemedicineAnimalsSympathomimeticsCyclic GMPCells Culturedomega-N-MethylargininebiologyChemistryGUCY1A3Guanylate cyclase 2CRatsEnzyme ActivationMethylene BlueNitric oxide synthaseEndocrinologyGuanylate Cyclasebiology.protein

description

Cyclic GMP (cGMP) formation in rat pinealocytes is regulated through a synergistic dual receptor mechanism involving beta- and alpha 1-adrenergic receptors. The effects of NG-monomethyl-L-arginine (NMMA), which inhibits nitric oxide (NO) synthase and NO-mediated activation of cytosolic guanylate cyclase, and methylene blue (MB), which inhibits cytosolic guanylate cyclase, were investigated in an attempt to understand the role of NO in adrenergic cGMP formation. Both NMMA and MB inhibited beta-adrenergic stimulation of cGMP formation as well as alpha 1-adrenergic potentiation of beta-adrenergic stimulation of cGMP formation, whereas they had no effect in unstimulated pinealocytes. The inhibitory action of NMMA was antagonized by addition of L-arginine. On the basis of these findings it can be concluded that the adrenergic stimulation of cGMP formation involves NO synthesis followed by activation of cytosolic guanylate cyclase.

yearjournalcountryeditionlanguage
1993-07-01Journal of Neurochemistry
https://doi.org/10.1111/j.1471-4159.1993.tb03548.x