6533b85efe1ef96bd12bf2f5
RESEARCH PRODUCT
Preparation, characterization and in vitro test of composites poly-lactic acid/hydroxyapatite scaffolds for bone tissue engineering.
Vincenzo La CarrubbaFrancesco Carfì PaviaGioacchino ConoscentiValerio BrucatoSilvia GrecoGiulio Ghersisubject
Thermogravimetric analysisMaterials scienceScanning electron microscopeCell SurvivalPolyestersComposite numberPolyesterBiocompatible Materials02 engineering and technologyMatrix (biology)010402 general chemistry01 natural sciencesBiochemistryBone and BonesHydroxyapatiteCell LineScaffoldMiceDifferential scanning calorimetryTissue ScaffoldTissue engineeringStructural BiologyMaterials TestingAnimalsMolecular BiologyMechanical PhenomenaBiocompatible Materialchemistry.chemical_classificationOsteoblastsCalorimetry Differential ScanningTissue EngineeringTissue ScaffoldsAnimalOsteoblastBiomarkerGeneral MedicinePolymer021001 nanoscience & nanotechnology0104 chemical sciencesPolyesterDurapatiteChemical engineeringchemistryThermogravimetry0210 nano-technologyPorosityBiomarkersBone and Bonedescription
Abstract In this work, the possibility to produce composite Poly-L-lactic acid (PLLA)/Hydroxyapatite (HA) porous scaffolds via Thermally Induced Phase Separation (TIPS) for bone tissue engineering applications was investigated. Several PLLA/HA wt/wt ratios (95/5, 90/10, 70/30, 50/50, 34/66) were tested and the as-obtained scaffolds were characterized via Scanning Electron Microscopy, Wide Angle X-Ray Diffraction, Thermogravimetric analysis, Gas Pycnometry, Differential Scanning Calorimetry and mechanical compression test. Morphological analysis revealed an open structure with interconnected pores and HA particles embedded in the polymer matrix. Finally, cell cultures were carried out into the composite scaffolds in order to evaluate the effect of HA on the proliferation and differentiation of osteoblastic cells, showing a higher alkaline phosphatase activity on composite scaffolds compared to neat PLLA ones.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2018-11-01 | International journal of biological macromolecules |