6533b85efe1ef96bd12bf347

RESEARCH PRODUCT

Impact of crop species on bacterial community structure during anaerobic co-digestion of crops and cow manure

Jaakko A. PuhakkaKatariina E.s. TolvanenHong WangAnnimari LehtomäkiJukka Rintala

subject

Crops AgriculturalEnvironmental EngineeringSilageBioengineeringcomplex mixturesClostridiaBioreactorsRNA Ribosomal 16SAnimalsAnaerobiosisWaste Management and DisposalPhylogenyElectrophoresis Agar GelBacteriaBase SequencebiologyRenewable Energy Sustainability and the Environmentfungitechnology industry and agriculturefood and beveragesBacteroidetesGeneral MedicineStrawequipment and suppliesbiology.organism_classificationManureClone CellsManureAnaerobic digestionAgronomyCattleSugar beetCow dungPolymorphism Restriction Fragment Length

description

The bacterial communities in three continuously stirred tank reactors co-digesting cow manure with grass silage, oat straw, and sugar beet tops, respectively, were investigated by 16S rRNA gene-based fingerprints and clone libraries. The analyses revealed both clearly distinct and similar phylotypes in the bacterial communities between the reactors. The major groups represented in the three reactors were Clostridia, unclassified Bacteria, and Bacteroidetes. Phylotypes affiliated with Bacilli or Deltaproteobacteria were unique to the sugar beet and straw reactor, respectively. Unclassified Bacteria dominated in sugar beet reactor while in the straw and grass reactor Clostridia was the dominant group. An increase in organic loading rate from 2 to 3kg volatile solids m(-3) d(-1) resulted in larger changes in the bacterial community in the straw compared to grass reactor. The study shed more light on the evolution of bacterial community during anaerobic co-digestion of different crops and manure to methane.

yearjournalcountryeditionlanguage
2008-03-28Bioresource Technology
https://doi.org/10.1016/j.biortech.2008.10.040