6533b85efe1ef96bd12bfac1

RESEARCH PRODUCT

Visualizing In Vitro Type I Collagen Fibrillogenesis by Transmission Electron Microscopy

J. Robin Harris

subject

0301 basic medicineChemistryfood and beveragesFibrillogenesisContext (language use)02 engineering and technology021001 nanoscience & nanotechnologyFibrilNegative stainIn vitrolaw.invention03 medical and health sciences030104 developmental biologyTransmission electron microscopylawBiophysicsElectron microscope0210 nano-technologyType I collagen

description

Techniques and protocols for the in vitro formation of collagen type I fibrils and the extensive biochemical variation of the fibrillogenesis conditions are presented. In all cases, the incubation and fibrillogenesis product can be readily monitored by transmission electron microscopic study of negatively stained specimens. Representative TEM data is presented and discussed within the context of the products of the fibrillogenesis protocols, from which the extensive biochemical and structural possibilities of this integrated approach can be appreciated.

yearjournalcountryeditionlanguage
2017-01-01
https://doi.org/10.1007/978-1-4939-7113-8_24