6533b85efe1ef96bd12bfd6d

RESEARCH PRODUCT

Anti-Proliferative Activity of A Hydrophilic Extract of Manna from Fraxinus angustifolia Vahl through Mitochondrial Pathway-Mediated Apoptosis and Cell Cycle Arrest in Human Colon Cancer Cells

Alessandro AttanzioMaria A. LivreaLuisa TesoriereAnna FrazzittaMario AllegraIgnazio Restivo

subject

Programmed cell deathCell cycle checkpointPharmaceutical ScienceCaspase 3anticancerAnalytical Chemistrylcsh:QD241-441<i>Fraxinus angustifolia</i>03 medical and health sciences0302 clinical medicinelcsh:Organic chemistrySettore BIO/10 - BiochimicaDrug DiscoverymedicineMTT assayPhysical and Theoretical Chemistry030304 developmental biologyFraxinus angustifolia0303 health sciencesChemistryOrganic ChemistryapoptosisSettore CHIM/08 - Chimica FarmaceuticaMolecular biologyChemistry (miscellaneous)Apoptosis030220 oncology & carcinogenesisCancer cellMolecular Medicinecolon cancer cellsMannitolIntracellularmannamedicine.drug

description

Manna is produced from the spontaneous solidification of the sap of some Fraxinus species, and, owing its content in mannitol, is used in medicine as a mild laxative. Manna is also a rich source of characteristic bio-phenols with reducing, antioxidant and anti-inflammatory properties. This study assesses the activity of a hydrophilic extract of manna (HME) on cellular and molecular events in human colon-rectal cancer cells. HME showed a time- and concentration-dependent anti-proliferative activity, measured by MTT assay, in all the cell lines examined, namely Caco-2, HCT-116 and HT-29. The amounts of HME that caused 50% of cell death after a 24 h treatment were 8.51 &plusmn

yearjournalcountryeditionlanguage
2020-10-30Molecules
https://doi.org/10.3390/molecules25215055