6533b85efe1ef96bd12bff8e
RESEARCH PRODUCT
Study of mycotoxin calibration approaches on the example of trichothecenes analysis from flour
Josep RubertJordi MañesCarla SolerKevin J. Jamessubject
FlourWheat flourAnalytical chemistryToxicologyMass spectrometryMatrix (chemical analysis)chemistry.chemical_compoundTandem Mass SpectrometryCalibrationControl materialExternal matrix-matched calibrationMycotoxinReference standardsChromatography High Pressure LiquidTriticumInternal standard calibrationChromatographyMass spectrometryMatrix matched calibrationGeneral MedicineReference StandardsMycotoxinsDeuteriumchemistryCalibrationIndicators and ReagentsTrichothecenesFood Sciencedescription
The aim of this study was to evaluate the applicability of different calibration approaches in trichothecenes analysis from wheat flour. Concretely eight trichothecenes (five type B trichothecenes and three type A trichothecenes) were analyzed by matrix solid-phase dispersion (MSPD) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). In the first set of experiments the presence of matrix effects was evaluated; values ranged between 59% and 79%. In the second set of experiments, solutions to compensate these signal suppressions were examined. Different calibration methods showed to tackle matrix effects obtaining values between 69% and 85% for external matrix matched calibration and values from 76% to 111% and from 78% to 108% by analog and deuterated internal standard calibrations, respectively. Trueness of the method was studied using quality control material and certificated reference material (DON 1062 ± 110 μg kg(-1)). The values were compared by matrix-matched, analog internal standard (deepoxydeoxynivalenol (DOM-1)) and deuterated internal standard (DON-d(1)).
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2012-01-01 |