6533b85efe1ef96bd12c056d
RESEARCH PRODUCT
Isolation and structural characterization of polypeptide antibiotics of the peptaibol class by high-performance liquid chromatography with field desorption and fast atom bombardment mass spectrometry
Michael PrzybylskiHans Brücknersubject
chemistry.chemical_classificationAlamethicinChromatographyOrganic ChemistryPeptaibolSequence (biology)General MedicineFast atom bombardmentMass spectrometryBiochemistryHigh-performance liquid chromatographyAnalytical ChemistryAmino acidchemistry.chemical_compoundchemistryField desorptiondescription
Abstract A number of polypeptide antibiotics of the peptaibol class, i.e., trichotoxin, alamethicin, suzukacillin, hypelcin and paracelsin, have been separated into components and isolated by high-performance liquid chromatography on spherical, porous octadecylsilyl bonded phases. All peptaibols were found to reveal a strong microheterogeneity due to single or multiple amino acid exchange. Most of the closely related and partially isobaric sequence analogue could be resolved using mixed alcohol—water eluents. As demonstrated by the structure analysis of the paracelsins and the main component of trichotoxin A-50, high-performance liquid chromatography with field desorption and fast atom bombardment mass spectrometry have been found to be a powerful and universally applicable method for the direct and unequivocal sequence determination of components of this class of polypeptides.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1984-07-01 | Journal of Chromatography A |