6533b85efe1ef96bd12c073c

RESEARCH PRODUCT

Characterization of two new cuticular genes specifically expressed during the post-ecdysial molting period in Tenebrio molitor

Jocelyne MathelinBrigitte QuennedeyHervé BouhinJean Delachambre

subject

Signal peptideMolecular Sequence DataGene ExpressionGenes InsectMoltingBiologyComplementary DNAGeneticsAnimalsGenomic libraryAmino Acid SequenceRNA MessengerTenebrioGeneHistidineMessenger RNAGenomeBase SequenceSequence Homology Amino AcidPupaIntronGene Expression Regulation DevelopmentalDNASequence Analysis DNAGeneral MedicineMolecular biologyGenesBiochemistryLarvaInsect ProteinsMoulting

description

Abstract In a previous study, we have isolated a cDNA, TM-ACP17 , coding for a post-ecdysial adult protein of Tenebrio molitor . After screening of a genomic library with TM-ACP17 , we report isolation and sequencing of TM-ACP17 gene and a new gene, TM-LPCP29 , coding for a larval–pupal protein. These two genes exhibit a common sequence of 15 nucleotides and a characteristic of most cuticular protein genes so far described: an intron interrupting the signal peptide. The deduced aa sequence of TM-LPCP29 exhibits a high percentage of Ala (26.5%) and Val (17.5%) and is highly hydrophobic. In the N-terminal part, the motif VAAPV is repeated ten times. Numerous histidine residues are present in the C- and N-terminal regions. A comparison is made with other cuticle protein sequences. Northern hybridization analysis showed that TM-LPCP29 is present during larval and mainly pupal post-ecdysial cuticle secretion. In-situ hybridization revealed that TM-LPCP29 mRNA is expressed in epidermis and not in muscles or fat body.

yearjournalcountryeditionlanguage
1998-05-01Gene
https://doi.org/10.1016/s0378-1119(98)00125-5